α-Amylase immobilization on gelatin: Optimization of process variables
نویسندگان
چکیده
منابع مشابه
Effect of different immobilization techniques on α- amylase
Immobilization has emerged since last decade as a very powerful tool to improve almost all enzyme properties like stability, activity, specificity and selectivity, and reduction of inhibition. The immobilization may help to solve some of the problems of enzymes as industrial biocatalysts like enzyme recovery for reuse. In the present investigation A.niger was isolated from soil and identified b...
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α-Amylase from Trichoderma harzianum was covalently immobilized on activated wool by cyanuric chloride. Immobilized α-amylase exhibited 75% of its initial activity after 10 runs. The soluble and immobilized α-amylases exhibited maximum activity at pH values 6.0 and 6.5, respectively. The immobilized enzyme was more thermally stable than the soluble one. Various substrates were hydrolyzed by imm...
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A commercial amylase (amy) was immobilized by adsorption onto Luffa operculata fibers (LOFs). The derivative LOF-amy presented capacity to hydrolyze starch continuously and repeatedly for over three weeks, preserving more than 80% of the initial activity. This system hydrolyzed more than 97% of starch during 5 min, at room temperature. LOF-amy was capable to hydrolyze starch from different sour...
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Immobilization of α-Amylase produced by a Pseudomonas sp isolated from Indian Chicken feather was studied. Partially purified enzyme with 486.77 IU and specific activity of 811.28 units mg (protein) was used for immobilization study. An easily available and inexpensive 3% Sodium alginate matrix with easy immobilization gel entrapment procedure was used for trapping the enzyme showed 555.5 IU ac...
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Immobilized metal chelate affinity chromatography (IMAC) support was practiced for α -amylase immobilization. Poly(hydroxyethylmethacrylate-methacryloylamidotryptophan)-Ni [p(HEMA-MAT)-Ni ] nanospheres, average diameter 100 nm, were produced by surfactant free emulsion polymerization. Characterizations of p(HEMAMAT)-Ni nanospheres were carried out by Fourier transform infrared (FTIR) spectrosco...
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ژورنال
عنوان ژورنال: Journal of Genetic Engineering and Biotechnology
سال: 2012
ISSN: 1687-157X
DOI: 10.1016/j.jgeb.2012.03.003